Effect of hydrogen sulphide containing mineral water on experimental osteoporosis in rats

Background/Aim: Sulphur mineral water is widely used in the treatment of musculoskeletal diseases. Hydrogen sulphide an important regulator bone metabolism and its application osteoporosis intensively researched. The aim this study was to analyse biochemical histological effects H2S containing "Mlječanica" spring on ovariectomy-induced experimental rats. Methods: In experiment a 14-week-old Wistar female rats were used. animals undergone bilateral ovariectomy (OVX groups) as model for oestrogen-deficient osteoporosis. After six weeks, divided into control group. Rats from group treated with (SW group) ad libitum during five weeks. Biochemical parameters monitoring sulphur concentration serum osteocalcin, alkaline phosphatase, calcium phosphorus. Histological analyses left tibia coloured haematoxylin-eosin carried out. Results: Regarding parameters, statistically significant increase observed OVX phosphatase phosphorus compared sham-operated (CNT) (p < 0.01). SW + OVX, significantly decreased 0.01) osteocalcin increased Calcium values without significance. group, showed numerous osteoblasts along trabecular endosteum growth young chondrocytes central zone their migration peripheral parts. Conclusion: Drinking has led stimulated reparation osteoporotic